Identification of early-stage diseases markers and a fast detection of markers accompanying disease development processes play a crucial role in modern personalized medicine. Preparation of nanofiber-based detectors functionalized on nanofiber surface with relevant antibodies belong among the key steps towards highly sensitive and specific bionanosensors. This Project is aiming to preparation of the novel specifically
functionalized nanochip a a part of the microfluidic diagnostic system and its testing on model fluids and body fluids. Bionanosensors based on functionalized nanofibers technology - equipped with selectively specific antibodies and/or miRNAs - allow the development of highly sensitive and specific test systems for
ultrasensitive detection of early-stage markers of pathological processes and are very suitable for smart active-targeted and controlled drug delivery systems. Especially important is the quick and highly specific diagnosis of pathological biomarkers predating post-prosthetic complications such as periprosthetic infection, periprosthetic osteolysis, and others. This study aims to develop and test bionanosensors for the detection of
specific biomarkers in human fluids (urine, synovial fluid, and others) for the early detection of developing periprosthetic complications. The main result of this study and the overall development of the application of
the bionanosensors in clinical practice would be the reduction of the number of complications, also the reduction of severe courses, and the overall reduction of the financial burden on the health care system.