The main objective of the project is the increase of the nystatin antibiotics production achieved by decreasing the variability of outcomes of the respective batches of the fermentation phase of the production. The means to achieve the objective is the design of knowledge-based control of fermentation processes as a supervisory level to the classical, current control system. Consecutively, after the process analysis, its mathematical and physiological models will be created, and based on these, the specific knowledge-based control strategy will be designed, which will bring savings of energy and input feeds, and will increase the yield of the fermentation process. The designed solution will be applied on the production of the nystatin antibiotics, while being easily transferable onto production of other antibiotics produced by the Streptomyces genus. As a result of the increased production, the increasing demand on the nystatin market will be satisfied.