Tissue engineering aims to regenerate damaged tissues employing bioactive scaffolds. The scaffolds should enable cell migration and provide suitable environment for cells. The aim of this project is reaserch in field of cartilage and bone tissue engineering employing centrifugal electrospinning as a novel method for production of three-dimensional nanofibrous scaffolds. Scaffolding material with higher pore size and loosly packed fibers are overcoming limitations of classically electrospun nanofibers and would efficiently support cell proliferation and vascularization. Moreover, in order to actively stimulate cell migration, proliferation and differentiation, we will study possibilities of coaxial nanofiber production by centrifugal electrospinning. Incorporation of stimulating molecules into the scaffolds and their controlled release would enable cell migration inside the scaffold and improve the cell proliferation and differentiation. The results of the project will bring new insight into mechanism of cartilage and bone regeneration in the field of tissue engineering.